The effects of adenosine on the energy metabolism of the
reperfused intestine in rats
Zhong Qiu Wang, Takuji Todani, Yasuhiro Watanabe, Akira Toki
Surg Today 28: 178-183, 1998
The effects of adenosine on energy metabolism in the intestine during reperfusion
after intestinal ischemia were examined in rats subjected to intestinal
ischemia for 60 min by clamping the superior mesenteric artery, followed
by 20 min reperfusion with tested agents. The rats were divided into a control
group, a 200 micro-g adenosine group, a 500 micro-g adenosine group, and
a 500 micro-g adenine group. Jejunal tissues were taken preischemia, 30
and 60 min post-ischemia, and 20 min after starting reperfusion. Adenosine
triphosphate, -diphosphate, -monophosphate, and thiobarbituric acid reactive
substances (TARS) of lipid peroxidation were measured by high-performance
liquid chromatography or spectrophotometry. The ATP Ievels in the jejunal
tissues decreased extensively 30 min after ischemia, but no further decrease
was observed 60 min after ischemia. These levels recovered slowly 20 min
after starting reperfusion in the control group, but they recovered significantly
in the 500 micro-g adenosine group and moderately in the adenine group,
with no significant difference between the 200 micro-g adenosine and control
groups. Thus, the effect of adenosine on energy metabolism appears to be
dose-dependent. The TARS Ievels increased significantly during ischemia
and reperfusion, but no significant difference was observed between the
control and 500 micro-g adenosine groups. In conclusion, adenosine promotes
the rapid resumption of ATP levels during reperfusion, but adenine is less
effective. Adenosine does not affect lipid peroxidation mediated by free
radicals.
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